SEPARATION OF YEAST HAVING HIGH PRODUCTIVITY OF FRAGRANT SUBSTANCE, MUTANT YEAST AND ITS USE

摘要

PURPOSE:To obtain the subject yeast giving a liquor rich in fragrant component owing to the high productivity of the fragrant component independent of the kind of fatty acid in the raw material by separating a mutant strain which has become unable to utilize the fatty acids originated from the raw material by mutation. CONSTITUTION:The objective yeast is obtained by carrying out the mutation of a yeast and separating a mutant which has become unable to utilize the fatty acids originated from the raw material. Concretely, the KYOKAI No.7 sake yeast belonging to Saccharomyces cerevisiae is subjected to mutation treatment with ethylmethane sulfonate and cultured in a medium incorporated with cerulenin (an antibiotic substance specifically inhibiting the synthesis of fatty acid) and palmitic acid (a fatty acid) and the colonies unable to grow are separated to obtain the new mutant Saccharomyces cerevisiae K-7-CF4 (FERM P-13177).