METHOD FOR THE COLORIMETRIC DETERMINATION OF AN ANALYTE WITH A PQQ-DEPENDENT DEHYDROGENASE

摘要

The invention concerns a method for the colorimetric determination of an analyte by means of enzymatic oxidation with a PQQ-dependent dehydrogenase in the presence of an electron acceptor from the group of the electron-rich aromatic nitroso compounds by enzymatic reduction of the nitroso compound to an imino compound and detection of the imino compound by colour formation. In addition the invention concerns a corresponding agent containing a PQQ-dependent dehydrogenase, an electonrich aromatic nitroso compound as well as, if desired, a detection reagent for imino compounds. The invention in addition concerns novel nitrosoamino compounds which form coloured quinone diimines on reduction as well as their use for the colorimetric enzymatic determination of an analyte.