HUMANIZED AND CHIMERIC MONOCLONAL ANTIBODIES THAT RECOGNIZED EPIDERMAL GROWTH FACTOR RECEPTOR (EGF-R). USE DIAGNOSTIC AND THERAPEUTIC

摘要

The invention relates to new humanized and chimeric monoclonal antibodies that recognized EGF-R comprising an artificial sequence at least of the FRs of the heavy chain variable region of a human inmunoglobulin . The hypervariableregions of both monoclonal antibodies is the following amino acid sequence: light chain: CDR-1:-arg-ser-ser-gln-asn-ile-val-his-ser-asn-gly-asn-thr-tyr-leu-asp;CD R-2:-lys-val-ser-asn-arg-phe-ser; CDR-3:-phe-gln-tyr-ser-hys-val-pro-trp-thr. Heavy chain: CDR-1:- asn-tyr-tyr-ile-tyr; CDR-2:-gly-ile-asn-pro-thr-ser-gly-gly-ser-asn-phe-asn-glu-lys-phe-lys-thr; CDR-3:-gln-gly-leu-trp-phe-asp-ser-asp-gly-arg-gly-phe-asp-phe. The antibodies can be used for therapeutical and diagnostic purposes.