PROBE FOR LIVING ORGANISM IDENTIFICATION

摘要

FIELD: biotechnology, genetic engineering, selection. SUBSTANCE: method involves the isolation of living organism DNA which after fragmentation and fractionation is subjected for hybridization with new probe as human DNA fragment (size is 485 nucleotide pairs). This DNA fragment has repeating trinucleotide TCC and shows the following structure: GATCTCGCTG GGAGCTGTAG ACCGGAGGTG TTCCTATTTG GCCATCTTGG AAGCGACTTC CTCCTTCCTT TTCTGAGTTT CAAAACTGTT TATATAGGTT GGGAATTTTA TTTTCTAAAA GTCTGAGTAA CTTATTTGTA AAACATTTCA AACCTGGAGG CTTTGGGGTA GATACTTCTC TTTCTCCTAT TTTTCCAAAG TTACTAATCC ATGCCAGTTG CAACAACTAG TTCTTCTCCT CCTCGTCCTC CTCCTCCTCC TCCTCCTCCT TCTTCTTCTG AGATGTATTC TCACTCTGTC ACTCAGGCTG GAGTCTCAGC TCACTTCAAC CTCTGCTCCT GGGTTCAAAC AATTCTGCCC CAGCCTCCAG TGTAGCTGGG ACTACAGGCA TGTGACACCA CTCCTGGATA CTTTTTTGTA TTTTTACTAG AGACAGGGTT TCACCATGTT AGCCAGGATG GTCTCAATCT CCTGACCTCG TGATC where: recognition site with restrictase Eco31I is at distance 460 nucleotide pairs from the left insertion end; recognition sites with restrictase GsuI are at distances 154 and 308 nucleotide pairs from the left insertion end; promoters and sites containing genes are absent. EFFECT: enhanced effectiveness of probe.