| 摘要 |
PURPOSE: To suppress a smiling phenomenon and to accurately determine a base sequence by preheating an electrolyte in which the end part of a migration gel on the injection side of a nucleic acid fragment sample is immersed at the start of migration. CONSTITUTION: At the start of the migration of a DNA sample, the electrolyte of an electrolytic cell 8 is preheated at about 40-60 deg.C by a heater 24. The heating of the electrolyte is started before the sample is introduced into the sample charging slot 14 provided to the upper end of a slab-shaped migration gel 2 and performed even during the introduction of the sample to be continued up to the start of migration. After the start of migration, the heating of the electrolyte may be continued or stopped. This heated electrolyte becomes a heat source and the temp. of the gel 2 at the part where the slit 14 is provided becomes uniform. By this constitution, the smiling phenomenon caused by the non-uniformity of temp. is suppressed and a base sequence of nucleic acid can be accurately determined. |
