BIOSENSOR AND METHOD FOR DETERMINING SUBSTRATE USING IT

摘要

PROBLEM TO BE SOLVED: To provide a reliable method for determining substrate by suppressing the error and scattering in a measurement result due to the selection of the electrode material of a reference electrode to be used and a manufacturing method when determining the substrate by a three-electrode-type disposable sensor. SOLUTION: In a method for determining a substrate by detecting a substance temperature concentration change when reacting enzyme to a substrate in a sample based on an electrochemical response obtained by applying a potential to a working electrode 5 using a biosensor with an electrode system having the working electrode 5, a counter electrode 8, and a reference electrode 7 made of silver formed on an insulation substrate 1 and a reaction layer containing at least enzyme, an anode potential is applied to the reference electrode 7 before applying a potential to the working electrode 5, thus causing reaction with a chloride contained in the sample or that provided at the sensor and manufacturing silver/silver chloride electrode on the reference electrode 7.