| 摘要 |
<p>PROBLEM TO BE SOLVED: To provide an excellent antibody having high specificity for 2'- deoxycytidine(dCyd) by transforming the 5-th or 3rd position hydroxyl group of dcyd into succinyl, dehydration-linking it with the amino group of carrier polypeptide via the carboxyl group, and using the dCyd antigen expressed by a formula. SOLUTION: 2'-deoxycytidine(dCyd) is transformed into succinyl by succinic anhydride, succinylated derivative at the fifth or third position is separated by HPCL, for example, and it is linked with carrier polypeptide or protein to prepare the dCyd antigen expressed by the formula I or II. An animal (mouse, cattle, horse) is immunized against this antigen, antibody forming cells are collected from the immunized animal and cell-fused with myeloma cells to form hybridoma, and an anti-dCyd monoclonal antibody is obtained. The dCyd in a specimen is reacted to the anti-dCyd antibody serving as a reagent, and the existence or abundance of dCyd in the specimen is detected from the reaction result.</p> |
