METHOD FOR SYNTHESISING SOLUBLE RECOMBINANT PROTEINS FROM BACTERIA CELLS

摘要

The invention relates to a method of manufacturing soluble recombinant proteins from bacteria, in which the plasmid contains the entire gene behind a promoter, this plasmid being expressed and the protein obtained being isolated and purified, in which the cell culture is raised to an OD 600 nm of 0.4 to 0.6; the cell culture is cooled over a period of 15 to 40 minutes to 20 to 26.degree.C; synthesis is induced by addition of 10 to 55 .mu.M of an inductor; and the induction phase is carried out at a temperature of 20 to 26.degree.C over a period of 3.5 to 6.5 hours.