Biosynthesis method enabling the preparation of cobalamins

摘要

In the biosynthesis of cobalamines (A) by fermenting an appropriate prokaryotic microorganism, the new feature is: (a) the microorganism is transformed with /- 1 DNA fragment encoding an enzyme involved in biosynthesis of O-phospho-L-threonine (I); (b) (I) is added to the culture medium or (c) aerobic producing organisms are transformed with /- 1 DNA fragment encoding an enzyme involved in biosynthesis of 5,6-dimethylbenzimidazole (II). Also claimed are microorganisms of (a) and (c).