MOLECULAR DIAGNOSIS OF VIRAL INFECTIONS

摘要

Reverse transcription employing pooled short oligonucleotides of random sequence as primers is used to obtain cDNA from viral RNA in a extract of cells suspected of being infected by RNA pathogens. The cDNA may be amplified in a single PCR procedure employing primers specific for two or more pathogens in order to detect the presence of multiple RNA pathogen infection in the cells. DNA so amplified may incorporate bromo-deoxyuridine which facilitates a convenient and non-radioactive means for detecting the amplified product.