| 摘要 |
<p>An instrument is disclosed for fluorescence assays which is capable of reading many independent samples at the same time; with multiple-beam source (41a-41d), mirror (42), samples (46a-46d) and detectors (45a-45d). This instrument provides enhanced throughput relative to single-sample instruments, time-resolved fluorescence, multi-band fluorescence, fluorescence resonance energy transfer (FRET), and fluorescence polarization. This invention is beneficial in applications such as high-throughput drug screening, and automated clinical testing. Also disclosed are means and methods for a fluorescence polarization measurement which is highly sensitive, inherently self-calibrated, and unaffected by lamp flicker or photobleaching. This fluorescence polarization invention can be practiced on a variety of fluorescence instruments, including prior-art equipment such as microscopes and multi-well plate readers.</p> |
