| 摘要 |
PROBLEM TO BE SOLVED: To obtain a newα1,4-N-acetylhexosmine transferase having bothα1,4-N-acetylgalactosamine transferase activity andα-1,4-N-acetylglucosamine transferase activity and useful for biosynthesis of glucosaminoglucans, their investigation or the like. SOLUTION: This newα1,4-N-acetylhexosmine transferase has bothα1,4-N- acetylgalactosamine transferase activity andα1,4-N-acetylglucosamine transferase activity, and has following enzymatic properties regarding activity and substrate specificity: GalNAc is transferred to the non-reductive terminal of a substance of formula I (GlcA is a galactose residue), formula II (Xyl is a xylol residue), formula III and formula IV (Cer is a ceramide residue) and is not transferred to a substance of formula IV from an N-acetylgalactosamine donor (GalNAc donor); and GlcNAc is transferred to a substance of formula I and not to a substance of formula II from an N-acetylglucosamine donor (GlcNAc donor).
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