MICROSCOPE SYSTEM

摘要

The purpose of the present invention is to detect a fixed number of photons or greater in a short time and to create a super-resolution image having a desired resolution equal to optical resolution or higher. A microscope system includes: a scanner that scans laser light emitted from a continuous-wave light source on a specimen; a beam splitter that splits fluorescence from the specimen on which the laser light is scanned into a plurality of light paths with the same wavelength; a plurality of PMTs that respectively detect the fluorescence in the light paths split by the beam splitter and output light intensity signals; a PC that, each time the laser light is repeatedly scanned by the scanner, for each of the PMTs, acquires an image dataset of the specimen on the basis of the light intensity signals, that combines the plurality of image datasets for the same area of the specimen, which are acquired on the basis of the light intensity signals of the fluorescence from the same area of the specimen, detected by each PMT, and that subjects this final combined image dataset to computational processing for enhancing high-frequency components.

主权项

1. A microscope system comprising:
a scanning unit that scans laser light emitted from a light source on a specimen; a light splitting part that splits return light from the specimen on which the laser light is scanned by the scanning unit into a plurality of light paths with the same wavelength; a plurality of photodetector units that respectively detect the return light in the light paths split by the light splitting part and output light intensity signals corresponding to the luminances of the return light; an image-dataset acquisition unit that, for each of the photodetector units, acquires an image dataset of the specimen on the basis of the light intensity signal; an image-dataset combining unit which, by repeatedly scanning the laser light with the scanning unit, combines the plurality of image datasets for the same area of the specimen, which are acquired by the image-dataset acquisition unit on the basis of the light intensity signals of the return light from the same area of the specimen, detected by the photodetector units for each scan; and a computational processing unit that subjects a final combined image dataset, obtained by combining the image datasets with the image-dataset combining unit, to image computational processing for enhancing high-frequency components.