ARRANGEMENT FOR ADJUSTING LASER OUTPUT AND/OR PULSE WIDTH OF SHORT-PULSE LASER IN MICROSCOPE

摘要

PROBLEM TO BE SOLVED: To combine nonlinear interaction with linear interaction and to make it adaptable to biological samples by detecting a nonlinear fluorescent signal and a reference signal corresponding to a reflection signal and/or a laser output, and by using a ratio between the two signals as the standard signal for adjustment. SOLUTION: As a linear signal in a detector D2, the reflection of a sample P illuminated by a laser beam L is detected through an objective lens O, main color splitter HFT and a sub-color splitter NFT. From this reflection signal, fluorescent radiation is separated using the sub-color splitter NFT, reaching a detector D1 through a short wave transmitting filter KP (a). Using it as a detector, the average output of the laser radiation is detected at a monitor diode MD (b). Then, by detecting a nonlinear fluorescent signal and a reference signal corresponding to a reflection signal and/or the average output of the laser radiation, the ratio between the two signals is used as the standard signal for adjustment.