| 摘要 |
PROBLEM TO BE SOLVED: To provide a substrate concentration determination method capable of determining the supply state of sample liquid easily and surely, and having high precision and little dispersion. SOLUTION: This substrate concentration determination method includes a process for judging the necessity of execution of a substrate concentration determination process, based on a period of time from the contact of the sample liquid with a working electrode 5 and a counter electrode 8 until the contact thereof with a liquid junction detection electrode 7, by using a biosensor in which the working electrode 5, the counter electrode 8, the liquid junction detection electrode 7, a reagent layer and a sample liquid supply opening 11 are provided and the liquid junction detection electrode 7 is arranged on the position separated from the sample liquid supply opening 11 furthermore than the working electrode 5 and the counter electrode 8. |
