| 摘要 |
PURPOSE: A method for producing silk-thread peptide is provided, thereby rapidly and cheaply producing the silk-thread peptide, which has a molecular weight of 500-10000 and 100% solubility at pH 4 to 9, in higher yield. CONSTITUTION: The method for producing silk-thread peptide comprises the steps of: inserting 1 part by weight of silk-thread protein into a solution containing 3 to 7 parts by weight of potassium chloride, 1.5 to 3.5 parts by volume(v/w) of ethanol, and 3 to 7 parts by volume(v/w) of water, and heating the solution at 85 to 95 deg. C for 1 to 3 hours to dissolve the silk-thread protein; dialyzing the silk-thread dissolved solution at 20 to 65 deg. C for 25 to 60 hours to remove salts; and adding 0.1 to 3.0%(w/v) of bromelain into 0.1 to 10.0%(w/v) of the silk-thread protein solution and reacting them at 25 to 30 deg. C for 1 to 5 hours. |
