| 摘要 |
PURPOSE: An androgen receptor complex-associated protein(ARCAP) is provided, which protein is overexpressed in hepatoma cells (relative to adjacent normal cells), and binds to an androgen receptor and augments the ability of the receptor to transactivate an androgen-responsive gene, so that liver cancer can be effectively diagnosed. CONSTITUTION: A pair of primers for detecting the expression of the human ARCAP gene consisting of a first primer and a second primer are provided, wherein the first primer contains SEQ ID NO: 2 and the second primer contains SEQ ID NO: 10, 11, 12, 13, 14, 15, or 16; the first primer contains SEQ ID NO: 3 and the second primer contains SEQ ID NO: 11, 12, 13, 14, 15, or 16; the first primer contains SEQ ID NO: 4 and the second primer contains SEQ ID NO: 12, 13, 15, or 16; the first primer contains SEQ ID NO: 5 and the second primer contains SEQ ID NO: 12, 13, 15, or 16; the first primer contains SEQ ID NO: 6 and the second primer contains SEQ ID NO: 12, 13, 15, or 16; the first primer contains SEQ ID NO: 7 and the second primer contains SEQ ID NO: 15 or 16; the first primer contains SEQ ID NO: 8 and the second primer contains SEQ ID NO: 16; the first primer contains SEQ ID NO: 9 and the second primer contains SEQ ID NO: 16; the first primer contains SEQ ID NO: 17 and the second primer contains SEQ ID NO: 11, 18, 12, 13, 14, 15, 16, or 19; the first primer contains SEQ ID NO: 20 and the second primer contains SEQ ID NO: 13, 14, 15, 16, or 19; the first primer contains SEQ ID NO: 21 and the second primer contains SEQ ID NO: 14, 15, 16, or 19; or the first primer contains SEQ ID NO: 22 and the second primer contains SEQ ID NO: 19; each primer being 20-40 nucleotides in length. A method of detecting liver cancer comprises the steps of: providing a sample from a subject, and determining a gene expression level of an androgen receptor complex-associated protein by amplification with a pair of primers or by hybridization with a nucleic acid amplified by using the primers, wherein the gene expression level in the sample, if higher than that in a sample prepared from a normal subject, indicates that the subject has liver cancer.
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