| 摘要 |
FIELD: biochemistry.SUBSTANCE: invention relates to biochemistry, namely to DNA construct for expression of genes in eukaryotic cells, where construct is linear DNA with open chain, containing promoter sequence, coding sequence and termination signal, where construct contains at least one L-DNA nucleotide and where at least one L-DNA nucleotide is located in last 2-5 nucleotides of 5′- and/or 3′- end, as well as to pharmaceutical composition containing it. Invention also relates to method of stable or transient transfection of human, animal or eukaryotic cells, which involves incubation of human, animal or eukaryotic cells with said DNA construct.EFFECT: invention enables efficient stable or transient transfection of human, animal or eukaryotic cells.10 cl, 7 dwg, 1 tbl |
