GENERATION OF CTL LINES WITH SPECIFICITY AGAINST MULTIPLE TUMOR ANTIGENS OR MULTIPLE VIRUSES

摘要

The present invention encompasses methods and compositions for the generation and use of cytotoxic T lymphocytes that target multiple viruses or that are specific for multiple tumor antigens. In specific embodiments, the generation methods employ use of certain cytokines to promote proliferation and reduce cell death in an activated T cell population and/or that employ a particular bioreactor having a gas permeable membrane.

主权项

1. A method of generating antigen-specific T lymphocytes that recognize at least two antigens from one virus, comprising the step of:
directly stimulating PBMCs with peptides, wherein said peptides comprise a sequence of amino acids spanning all or part of at least two viral antigens from the one virus, thereby producing antigen-specific T lymphocytes capable of recognizing a cell that expresses part or all of at least one of said viral antigens.