摘要 |
A high-throughput method for identifying a nucleic acid molecule of interest, said method comprising the steps of: (a) transforming a duckweed plant culture, duckweed nodule culture, duckweed suspension culture, or duckweed protoplast cell culture with a test nucleotide sequence comprising a coding sequence for a polypeptide; (b) culturing the duckweed plant culture, duckweed nodule culture, duckweed suspension culture, or duckweed protoplast cell culture under conditions such that the polypeptide encoded by the test nucleotide sequence is expressed; and (c) determining whether the polypeptide encoded by the test nucleotide sequence modulates a biological response, wherein a test nucleotide sequence that encodes a polypeptide that modulates a biological response is identified as a nucleic acid molecule of interest; wherein steps (a) and (b) are performed in the same high density format plate or container.
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