| 摘要 |
Provided is a vector for transferring and integrating a desired polynucleotide into the genome of at least one cell of a plant via bacterial-mediated transformation means, comprising: (1) a first polynucleotide, comprising the consensus sequence [A/C/G] - [A/C/T] - [A/C/T] - [G/T] - A- [C/G] - NNNNNN - A [G/T] - A - [A/C/T] - [A/G] - TCCTG - [C/G/T] - [A/C/G] - N, which is (i) nicked when exposed to an enzyme involved in bacterial-mediated plant transformation and (ii) not identical to a bacterial border sequence; (2) a second polynucleotide, which is a bacterial T-DNA border; and (3) a desired polynucleotide positioned between the first and second polynucleotides. Further provided is the method of transforming plants with the vector.
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