Microarray based sample detection system

摘要

A microarray assembly for detection of a target molecule is disclosed. The microarray assemblies comprise an array chamber having a microarray located therein and features that facilitate liquid movement within the array chamber. Also disclosed are methods for making the microarray assembly using rollable films and methods for detecting microarray spots using an internal control fluorophore in the array spot.

主权项

1. A microarray assembly for detection of a target molecule in a sample, comprising:
an array chamber with a sample inlet, a sample outlet, a top interior surface, a bottom interior surface, side walls and a microarray located on the bottom interior surface, wherein the array chamber is designed to allow amplification of nucleic acids in the array chamber and hybridization of amplified nucleic acid to the microarray and wherein the top interior surface is a hydrophilic surface that facilitates complete filling of the array chamber by an aqueous fluid; a waste chamber comprising a waste inlet and an absorbent material; and a channel having an expansion section with a first end proximate to the outlet of the array chamber and a second end proximate to the inlet of the waste chamber, wherein the expansion section of the channel has a reversed funnel shape from the first end to the second end, wherein the cross-sectional area of the channel increase continuously in a stepwise manner from the first end of the expansion section to the second end of the expansion section.