Automated generation of genetically modified stem cells

摘要

The invention relates to a process for generation of genetically modified stem cells comprising the steps
a) providing a cell sample in suspension comprising stem cells in a centrifugation chamber comprising a base plate and cover plate connected by a cylinderb) adjusting the volumetric concentration of stem cells in the cell sample to at least 1×105 stem cells per mL cell sample by centrifugationc) introducing viral and/or non-viral vectors to the centrifugation chamber for genetically modifying the stem cellsd) adjusting the spatial concentration of stem cells in the centrifugation chamber by rotating the centrifugation chamber at a speed where the cell sample is located at the outermost 35% of the radius of the base plate of the centrifugation chamber, thereby inducing gene modification of the stem cells.

主权项

1. A process for generation of genetically modified stem cells comprising the steps:
a) providing a cell sample in suspension comprising stem cells in a centrifugation chamber comprising a base plate and cover plate connected by a cylinder; b) adjusting the volumetric concentration of stem cells in the cell sample to at least 1×105 stem cells per mL cell sample by centrifugation; c) Introducing viral and/or non-viral vectors to the centrifugation chamber for genetically modifying the stem cells; and d) adjusting the spatial concentration of stem cells in the centrifugation chamber by rotating the centrifugation chamber at a speed where the cell sample is located at the outermost 35% of the radius of the base plate of the centrifugation chamber, thereby inducing gene modification of the stem cells.