| 摘要 |
<p>1,133,958. Distemper vaccine. BEHRING- WERKE A.G. 17 Jan., 1966 [19 Jan., 1965], No. 2088/66. Heading A5B. A distemper vaccine for mustelines is prepared by attenuating a distemper virus by at least 50 successive passages in dog tissue cell cultures, subjecting the attenuated virus to at least 10 successive passages in cultures containing musteline tissue cells and nutrient solution, and preparing a vaccine from the resulting virus suspension. Suitable cells are from the kidney epithelium of the dog, and the same organ, or spleen, testicles or uterus of mustelines such as ferrets, minks, martens, ermines, weasels, polecats or sables. Up to nine harvests of virus may be taken from the musteline cell culture, which consists of trypsindispersed tissue in nutrient medium. The virus may be subjected to secondary culture by dispersing the cells of a well developed infected primary culture, sedimenting the cells and resuspending in fresh nutrient solution for further cell growth and virus replication. The vaccine is prepared by addition to the virus suspension of gelatin broth and glucose at pH 7.6, optionally with an adjuvant such as aluminium hydroxide, followed by lyophilisation. The reconstituted vaccine may be administered by injection or as an inhaled spray.</p> |
