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1503547 Immunological reagent TAKEDA CHEMICAL INDUSTRIES Ltd 8 July 1975 [8 July 1974] 28759/75 Heading G1B [Also in Division C3 ] Latex reagents for serological reactions comprising latex particles on which there is adsorbed a polyoxyethylene non-ionic emulsifier and which have been sensitized with a serologically active substance, may be prepared by treating latex particles with the surfactant and with the serologically active substance. The latex particles may comprise natural rubber or synthetic polymers of styrenes, olefins, acrylic monomers, dienes, vinyl chloride, vinylidene chloride or vinyl acetate. The surfactant may be polyethylene oxide, polyoxyethylene alkyl or alkaryl ether, or an ethylene oxide/propylene oxide block copolymer. The latex particles may be sensitized by treating with an antigen or antibody in aqueous medium, e.g. water, physiological saline or buffer solution, at 20 to 37‹C at pH 7À0 to 8À6, optionally with stirring or shaking, and residual latex particle surfaces may be saturated by adsorbing bovine or equine serum albumin thereon. Examples describe the addition of ethylene oxide/propylene oxide block copolymers to latices of polystyrene and polyvinyl chloride, mixed lauryl and myristyl polyoxyethylene ethers to latices of polystyrene and polymethyl methacrylate and polyoxyethylene nonylphenyl ethers to latices of styrene/chlorostyrene copolymers; and the sensitizing of polystyrene latices with human immunoglobulin G, polysaccharide antigens from saliva, Australia antigen, anti-human haptoglobin precipitin serum and rabbit immunoglobulin G.
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