Enzymes immobilized on chitosan

摘要

An enzymatically active product insoluble in aqueous medium is prepared by treating chitosan as an inert support with a dialdehyde, after which an enzyme is fixed to the support thus treated. The chitosan may be in powder-form or in flake-form, for example obtained by fragmenting a film of anhydrous chitosan. The enzymatically active product insoluble in aqueous medium may be treated with a compound containing an amino group and/or with a reducing reagent. The product may be freeze dried optionally in the presence of twice its weight of mannitol.