System and method of non-reductive β-elimination isolation and analysis

摘要

A laboratory method and system used to isolate and analyze O-linked oligosaccharides from glycoproteins that uses non-reductive β-elimination (NBRE). The method including the step of providing a predetermined amount of solution and then passing it through an ion exchange resin. The method further includes collecting a second solution off of the ion exchange resin and adding a predetermined amount of sodium hydroxide to form a third solution. The third solution is allowed to stand for a set amount of time at a particular temperature. The third solution is then washed through an ion exchange cartridge in the ammonium form. The collected fourth solution is evaporated and pushed through a sodium form resin prior to being analyzed for its composition.

主权项

1. A method of non-reductive β-elimination isolation and analysis comprising the steps of:
providing a predetermined amount of a first solution; passing the first solution through an cation exchange resin and collecting a second solution off the cation exchange resin; adding a predetermined amount of sodium hydroxide to the second solution to form a third solution; letting the third solution stand for a predetermined amount of time; washing the third solution through an ion exchange cartridge and collecting a fourth solution off the ion exchange cartridge.