Ethanol dependence of alpha1 antitrypsin C-terminal Lys truncation by basic carboxypeptidases

摘要

Methods of preparing alpha-1-antiproteinase inhibitor and controlling the amount of des-lys alpha-1-antiproteinase inhibitor in the preparation, and compositions comprising the same, as well as methods of treatment using the same are provided.

主权项

1. A method of making an alpha-1-proteinase inhibitor composition comprising des-lys alpha-1 proteinase inhibitor from human plasma, the method comprising the step of:
treating a plasma precipitate comprising alpha-1-proteinase inhibitor with ethanol at a concentration of at least 5% and less than 15%, wherein the plasma precipitate is selected from the group consisting of a Cohn fraction IV-1 precipitate and a Cohn fraction IV-1+IV-4 precipitate.