GENERATION OF LIGATION-READY DNA AMPLICONS

摘要

The invention is directed to novel methods, kits and uses to be employed for the generation of ligation-ready DNA amplicons of a target DNA by using 5′-phosphorylated primers.

主权项

1. A method for generating ligation-ready DNA amplicons of a target DNA, comprising
(i) contacting in a polymerase chain reaction (PCR) buffer said target DNA with at least one DNA polymerase, a dNTP mixture, and at least one PCR primer pair consisting of two target specific PCR primers, to obtain a reaction mixture, (ii) subjecting said reaction mixture to a PCR to generate a plurality of ligation-ready DNA amplicons of said target DNA,  wherein at least one of said target specific PCR primers is 5′-phosphorylated.