Method for the determination of an antigen in solution.

摘要

An improvement in methods for the determination of an antigen (I) in solution, in which determination said antigen (I) is reacted with an antibody (II), which is directed against the antigen (I), and with an antibody (III), which is directed against the antigen (I) and is labelled with an analytically indicatable atom or group and is soluble in the liquid in the presence of which the determination is carried out, to the formation of a conjugate comprising said antigen (I) and said antibodies (II) and (III), which conjugate is insoluble or is made insoluble, whereafter the analytically indicatable atom or group is determined in the insoluble or insolubilized conjugate and/or in the solution. The improvement comprises using as antibody (II) and antibody (III) in said determination antibodies which are monoclonal and react with roomly spaced determinants of the antigen (I).