| 发明名称 | Characterization of CHO-MIF gene and protein, and use thereof | ||
| 摘要 | The present invention is concerned with the specific and highly sensitive detection of specific CHO-MIF (macrophage migration inhibitory factor from Chinese Ovarian Hamster cell line) complexes in the production of anti-MIF antibodies. The present invention is further concerned with the provision of specific antibodies which can be used for a CHO-MIF detection method. The present invention is also concerned with a CHO MIF knockout cell line and use thereof. The present invention also provides preparations obtained from recombinant production in CHO cell lines which are essentially free of CHO-MIF. | ||
| 申请公布号 | US9465037(B2) | 申请公布日期 | 2016.10.11 |
| 申请号 | US201214350187 | 申请日期 | 2012.10.04 |
| 申请人 | Baxalta Incorporated;Baxalta GmbH | 发明人 | Kerschbaumer Randolf J.;Voelkel Dirk;Antoine Gerhard;Scheiflinger Friedrich;Mudde Geert C. |
| 分类号 | C12N5/10;C12N5/16;C12N15/12;C12N15/63;G01N33/53;G01N33/68;C07K16/24 | 主分类号 | C12N5/10 |
| 代理机构 | Morgan, Lewis & Bockius LLP | 代理人 | Morgan, Lewis & Bockius LLP |
| 主权项 | 1. A method for the detection of Chinese hamster ovary (CHO)-macrophage migration inhibitory factor (MIF) contaminations in a monoclonal anti-MIF antibody preparation, the method comprising: i) contacting the anti-MIF antibody preparation with a polyclonal anti-CHO-MIF antibody, affinity purified against CHO-MIF, and ii) detecting the presence of CHO-MIF. | ||
| 地址 | Bannockburn IL US | ||