Compositions, methods, and kits for identifying candidate molecules from encoded chemical libraries

摘要

The subject matter relates to relates to a one-bead-one-sequence composition, a library of tagged chemicals comprising a plurality of one-bead-one-sequence compositions, a method for identifying a candidate molecule from a library of tagged chemicals, and a composition produced by a process, all as described herein.

主权项

1. A one-bead-one-sequence composition comprising:
a) a microscopic bead; b) a plurality of identical copies of a single-species, sequenceable molecule produced by at least one of BEAMing PCR or bridge amplification, each operably connected to said microscopic bead; and c) a tag sequence comprising DNA, modified DNA or RNA, which tag sequence is complementary to, and is hybridized to, said sequenceable molecule, d) a candidate chemical operably connected to said tag sequence, wherein said sequenceable molecule is a unique identifier for its complementary tag sequence, and wherein said tag sequence comprising DNA, modified DNA or RNA uniquely identifies and is directly connected to said candidate chemical.