发明名称 |
FREMGANGSMÅDE TIL CELLESEPARATION |
摘要 |
The present invention provides methods and compositions for separating cells from a sample containing erythrocytes. The method is for recovering desired cells from a sample containing the desired cells, erythrocytes and undesired cells comprising: a) contacting the sample with a composition, said composition comprising: i) an erythrocytes aggregation reagent ii) at least one antigen recognizing moiety coupled to a magnetic particle, wherein said particle with said at least one antigen recognizing moiety specifically binds to at least one antigen specific for one or more undesired cellular components; b) applying simultaneously i) gravity sedimentation for sedimentation of erythrocytes and ii) a magnetic field gradient to said sample for immobilizing said magnetic particle generating a pellet and a supernatant phase, and c) recovering the desired cells from the supernatant phase. Compositions for the use within the present method are also disclosed. |
申请公布号 |
DK2597153(T3) |
申请公布日期 |
2016.12.05 |
申请号 |
DK20110190850T |
申请日期 |
2011.11.25 |
申请人 |
Miltenyi Biotec GmbH |
发明人 |
Kauling, Burgund;HUPPERT, Volker |
分类号 |
B03C1/01;B01D21/00;B01D21/26;B03C1/28;C12N5/00;C12Q1/02;G01N1/34 |
主分类号 |
B03C1/01 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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