método para introduzir enzima celulase em pasta de matéria-prima lignocelulósica

摘要

The present invention provides a method for producing glucose from a lignocellulosic feedstock. The method comprises moving a pretreated feedstock slurry that has an undissolved solids content of between about 15 and about 30 wt % through a pipe. Cellulase enzyme is added to the pretreated slurry to produce a slurry comprising cellulase enzyme. A high shear in-line mixing device is used to disperse the cellulase enzyme in the pretreated slurry to produce a pretreated slurry comprising dispersed cellulase enzyme. The pretreated slurry comprising dispersed cellulase enzyme is then subjected to hydrolysis so as to produce glucose from cellulose contained therein.