Heat shock process for the isolation of bacterial protein

摘要

There is disclosed an improved process for the isolation of K-99 outer membrane protein pili from Escherichia coli cells. The improved process involves a heat shock treatment of the E. coli cells in a buffered solution to afford a release of the K-99 pili into solution which on standing results in the aggregation and precipitation of the pili. The thus isolated K-99 protein pili is useful in the preparation of vaccines.