发明名称 |
Multi-layer immobilized enzyme compositions |
摘要 |
A high activity immobilized enzyme composite is prepared by covalently bonding a second enzyme layer to a first enzyme layer that is immobilized to a carrier. The carrier is preferably silica gel which has been activated by treatment with a strong base followed by treatment with a strong acid. The first enzyme layer is covalently bonded to the activated silica gel with an aminosilane and a polyfunctional reactant, and the second enzyme layer is covalently bonded to the first layer with a polyfunctional reactant. Third, fourth and more successive enzyme layers may be covalently bonded. The composite has high activity per unit volume, superior stability and good half-life.
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申请公布号 |
US4506015(A) |
申请公布日期 |
1985.03.19 |
申请号 |
US19820435218 |
申请日期 |
1982.10.19 |
申请人 |
BORDEN COMPANY LIMITED |
发明人 |
HO, GUAN-HUEI;LIAO, CHIANG-CHANG |
分类号 |
C12N11/14;C12N11/18;C12P19/24;(IPC1-7):C12N11/18;C12N9/38;C12N9/92 |
主分类号 |
C12N11/14 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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