EUKARYGOTIC HYBRID VECTORS AND PREPARATION OF POLYPEPTIDES

摘要

Extrachromosomal ribosomal DNA (rDNA) can be used as a vector for introducing a foreign gene into eukaryotic cells. Eukaryotic hybrid vectors comprising an extrachromosomal rDNA or functional fragments thereof and a foreign structural gene coding for a polypeptide, and methods for their preparation, characterized in that a foreign structural gene coding for a polypeptide and optionally additional DNA sequences are introduced in vitro or in vivo into an extrachromosomal rDNA or functional fragments thereof. A eukaryotic cell transformed with a hybrid vector and methods for the preparation thereof, characterize in that a transformable eukaryotic cell is treated with the eukaryotic hybrid vector under transforming conditions.