| 摘要 |
Cloning vectors are described which include the streptomycin resistance (Smr) determinant derived from Tn904. A single site for the restriction endonuclease, AvaI, is present within the Tn904 determinant for Smr. A method is described for preparing the Tn904 containing cloning vectors through transposition of Tn904 to a parent cloning vector and then cloning of the Smr gene into another vector segment. The cloning vector is important for inserting deoxyribonucleic acid segments, which encode for various characteristics such as chemical production, antibiotic resistance or bacterial cell properties, in the Smr gene AvaI cleaved site and which normally provides a marker for identification of transformed strains of bacteria.
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