Monoclonal antibody in relation to drug-resistant cancers and production thereof.

摘要

By… (a) immunizing a mouse with an adriamycin-resistant K562/ADM strain which has been established from a human myelogenous leukemia cell K562 strain (ATCC CCL243);… (b) taking spleen cells out of the immunized mouse and preparing a dispersion of the cells;… (c) subjecting the spleen cells together with mouse myeloma cells to cell fusion conditions thereby to prepare a hybridoma as a fusion product between the spleen cell and the myeloma cell;… (d) culturing the mixture of cells obtained from the step (c) on a selective medium on which only the hybridoma can grow;… (e) determining whether the hybridoma-containing supernatant of the medium contains the antibody desired thereby select hybridoma capable of producing the antibody desired;… (f) cloning the hybridoma thus selected; and… (g) incubating the clone in an abdominal cavity of a mouse or on a medium a monoclonal antibody is formed and accumulated in the cancerous ascites or the supernatant of the medium.