Method for a highly sensitive detection and quantification of biomolecules using secondary ion mass spectrometry (SIMS) and related technologies

摘要

The present invention relates to a method for detecting and quantifying the presence or absence of a number of biomolecules in a sample using the SIMS technique and arrays for use in said method.

主权项

1. A method for detecting and quantifying in at least one sample the presence or absence of at least one biomolecule, comprising:
(a) contacting said at least one sample with an array which comprises a substantially planar substrate having a conducting surface and a number of discrete areas containing probes being labeled with at least one rare, stable or unstable isotope or exogenous isotope; (b) washing and drying the array; (c) detecting and counting by dynamic secondary ion mass spectrometry (D-SIMS) the common secondary ions along with the corresponding rare secondary ions; and (d) detecting and quantifying in at least one sample the presence or absence of at least one biomolecule.