| 摘要 |
The field of the present disclosure relates to plant molecular biology and, in particular, to constructs and methods to down-regulate the expression of target genes. Described herein are isolated nucleic acid fragments encoding artificial miRNAs and their use in down-regulating gene expression. In one aspect of the present disclosure, an artificial miRNA precursor is achieved by modifying the deoxyribonucleotide sequence that encodes a soybean miRNA precursor. In this aspect, the miRNA and star sequences of the miRNA precursor are replaced with variable nucleotide subsequences designed to down-regulate the expression of a target gene. In another aspect of the present disclosure, a plant cell is transformed with a nucleic acid construct comprising a nucleic acid fragment that encodes an artificial miRNA precursor for reducing the expression of a target gene. |
