Hybrid plasmids having a streptomycetes replicon and an escherichia coli replicon

摘要

Starting from the Streptomycetes plasmids pSG2, pSG5 and pSVH1, "shuttle vectors" which, in additon to a E. coli replicon, also contain suitable selection markers for E. coli and Streptomycetes are constructed. These shuttle vectors replicate both in E. coli and in Streptomycetes, and they can be used for cloning and expression of foreign DNA.