发明名称 |
DNA expression vector and use thereof |
摘要 |
A gene having a DNA sequence complementary to that of the glucoamylase polypeptide mRNA from a fungal species, preferably Aspergillus awamori, is prepared. The mRNA is an approximately 2.2 kilobase poly A RNA obtained from fungal cells grown under conditions of glucoamylase induction. Reverse transcription of the mRNA provides a glucoamylase probe used to identify genomic digest fragments containing glucoamylase gene regions, which are sequenced to locate the introns and exons. The genomic fragments are spliced together to form a gene having a DNA sequence with altered or deleted introns which codes for fungal glucoamylase protein and is capable, when correctly combined with a cleaved DNA expression vector, of expressing a non-native protein having glucoamylase enzyme activity upon transformation of a host organism by the vector. The host is preferably bacteria or yeast. The transformed yeast host may be used to produce ethanol.
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申请公布号 |
US5045463(A) |
申请公布日期 |
1991.09.03 |
申请号 |
US19880287688 |
申请日期 |
1988.12.19 |
申请人 |
CETUS CORPORATION |
发明人 |
INNIS, MICHAEL A.;GELFAND, DAVID H.;MEADE, JAMES H. |
分类号 |
C12N9/34;C12N15/10;C12N15/62;C12N15/70;C12N15/81;C12P7/06 |
主分类号 |
C12N9/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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