发明名称 |
Pheromone - inducible yeast promoter |
摘要 |
A yeast promoter inducible by the appropriate pheromone and a method of expressing a gene of interest in substantial quantities by placing it under the control of the inducible promoter. DNA encoding a protein of interest is fused or linked to a pheromone - inducible yeast promoter, such as the FUSI or the FUS2 promoter, and the fusion is inserted onto a high copy vector; the resulting product is introduced into wild type yeast cells. Stimulation of these yeast cells by the appropriate pheromone results in induction of transcription of the yeast promoter and expression of the protein of interest in substantial quantities.
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申请公布号 |
US5063154(A) |
申请公布日期 |
1991.11.05 |
申请号 |
US19880212270 |
申请日期 |
1988.06.24 |
申请人 |
WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH |
发明人 |
FINK, GERALD R.;TRUEHEART, JOSHUA;ELION, ELAINE A. |
分类号 |
C07K14/395;C12N1/19;C12N9/38;C12N15/81 |
主分类号 |
C07K14/395 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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