DETERMINATION OF HIGH MOLECULAR WEIGHT ANALYTES

摘要

MCRO-022/00 US DETERMINATION OF HIGH MOLECULAR WEIGHT ANALYTES A method for detecting the presence of a highmolecular-weight analyte, which uses a variation on complementation assays, comprising (1) providing an enzyme donor (ED)complex comprising ED coupled to an analyte-specific binding molecules wherein the ED complex retains measurable complementation activity such that active .beta.-galactosodase formed in the presence of an enzyme acceptor (EA); (2) contacting the ED complex and EA in the presence of a sample suspected of containing a high-molecular-weight analyte that reacts specifically with the analyte specific binding molecule; and (3) relating the presence of the analyte in the sample to the formation of active .beta.-galactosidase enzyme. 20216638 26