| 摘要 |
<p>An oligonucleotide wherein at least one nucleotide unit includes a phosphonate moiety having the structural formula (I) , wherein X is (a), R1 is a hydrocarbon, preferably methylene, and each of R2, R3, and R4 is hydrogen or a hydrocarbon, and each of R2, R3, and R4 may be the same or different. Preferably, X is an aminomethyl moiety. Such oligonucleotides having improved binding capabilities and improved resistance to nucleases. Alternatively, X may be (b) or (c), wherein R1, R2, and R3 are as hereinabove described, and R5 is a detectable marker, thus making such oligonucleotides useful as diagnostic probes.</p> |
