METHOD FOR THE DETECTION OF MUTATIONS AND MONOCLONAL LINEAGES BY ANALYSIS OF RNA CONFORMATION POLYMORPHISM(S)

摘要

The invention is a method to detect a mutation at a predefined position within a predetermined DNA sequence from a subject. The method comprises amplifying and transcribing the predetermined DNA squence in a sample from the subject and comparing the pattern of RNA bands in a non-denaturing gel with the RNA band pattern of the same predetermined DNA sequence absent the mutation wherein any differences detected in the compared patterns indicate the presence of mutation at the predefined position in the predetermined DNA sequence. The invention also provides a method for detecting a clonespecific DNA sequence at a predetermined position in a monoclonal lymphoid population of cells from a subject, the method comprising amplifying and transcribing the DNA containing the predetermined position in a sample from the subject and comparing the RNA band pattern in a non-denaturing gel with the pattern of bands of RNA transcribed from the DNA sample known to contain the predetermined position without the clonespecific sequence wherein the detection of differences in the compared band patterns indicates the presence of the monoclonal lymphoid population of cells.