VERFAHREN ZUM SCREENEN VON SUBSTANZEN MIT MODULIERENDER WIRKUNG AUF EINEN REZEPTORABHAENGIGEN ZELLULAEREN SIGNALUEBERTRAGUNGSWEG

摘要

A process is disclosed for screening substances having a modulating effect on a receptor-dependent signal transmission path in mammal cells. Test cells are used transformed with a reporter gene and with a regulatory sequence functionally linked thereto sensitive to the IP3/DAG concentration, as well as with a coding DNA for a receptor coupled to the phospholipase effector system, in particular a G protein-coupled receptor. The use of reference cells without receptor ADN and reference cells with specificity for the adenylate cyclase effector system allows substances to be identified having potential pharmacological action and specificity for a determined receptor-dependent signal transmission path.