Cell line and assay to determine biological activity of retinoids

摘要

The present invention relates to an assay and cell lines for determining the biological effects of retinoids. The cell line is derived from a human lung tumor line which is tumorigenic in nude mice and expressed undetectable levels of the beta receptor of retinoic acid by introducing in a stable fashion the DNA coding for this receptor. The novel line is non-tumorigenic and grows in culture at a rate which is reduced in the presence of 10<-7> M retinoic acid. This line is useful in determining the biological effects of novel retinoids which act in particular by activating RAR beta .