Recombinant ctla4 polypeptides and methods for making the same

摘要

The present invention relates to recombinantly-produced CTLA4 polypeptides having B7 binding activity that are not the product of a fusion between CTLA4 and human Ig genes. The CTLA4 polypeptides of the present invention generally contain an amino acid sequence corresponding to the extracellular domain of the CTLA4 receptor protein. The invention further provides functional derivatives of the CTLA4 polypeptides, including muteins and conjugates containing polyethylene glycol. Methods for preparing the recombinant CTLA4 polypeptides are also provided, as well as methods for separating the monomeric and dimeric forms of the recombinant polypeptides as well as active and less active dimer forms.